ANTI-CASPASE 12 ANTIBODY NT (C15-1316-115)

General description

Three distinct signaling pathways lead to apoptosis. The death receptor and mitochondrion pathways are the two mains, which involve respectively caspase-8 and caspase-9. The endoplasmic reticulum stress is the third apoptotic pathway and involves caspase-12. Localized to the ER but not to cytoplasm or mitochondria, caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer′s disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues.

Specificity

Antibody recognizes caspase 12 pro-protein in mouse extracts of normal and apoptotic cells. Caspase 12 is predicted to have a large >120aa long prodomain region. Thus this antibody recognizes only the proforms of the enzyme and not the active subunits. Uncleaved (proform) caspase 12 is prominently detected in many tissues. Active forms are difficult to detect expect in transfect cell systems. {Kalai, M et al (2003) 162:457-467}.

Immunogen

Epitope: N-terminus

Peptide corresponding to aa 2-17 of mouse caspase-12.

Application

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

This Anti-Caspase 12 Antibody, N-terminus is validated for use in WB for the detection of Caspase 12.

Western blot: 1:500 to 1:1000. Detects a band at ~53-49 kDa. in normal tissue extracts. Caspase 12 is a stress induced caspase localized to the ER and activated upon ER stress. Active process forms are difficult to detect in most non-transfected systems. Not all cells produce caspase 12; mouse L929 fibrosacrcoma cells are an excellent positive control line.

Mouse brain tissue lysate can be used as a positive control as well. Caspase 12 is associated with ER membranes and not in cytoplasm or mitochondria. Caspase 12 mediates cytotoxixity induced by amyloid beta.

Immunohistochemistry: 10μg/mL

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Storage and Stability

Maintain at 4°C for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse, rat. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. UniProt accession no.: Q6UXS9. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CASP12(100506742)
mouse ... Casp12(12364)
rat ... Casp12(156117). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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