General description
Caspase-5 (CASP5) is a member of the peptidase C14A family that can be divided into the apoptotic and inflammatory caspase subfamilies. Members of this subfamily include caspase-1, -4, -5, and -12. When activated, these caspases result in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18. Caspase-5 is expressed in barely detectable amounts in most tissues except brain, with highest levels being found in lung, liver and skeletal muscle. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts.
Specificity
This antibody recognizes the intermediate domain of Caspase-5.
Immunogen
Epitope: Intermediate domain
KLH-conjugated linear peptide corresponding to the intermediate domain of human Caspase-5.
Application
Detect Caspase 5 using this rabbit polyclonal antibody, Anti-Caspase-5 Antibody validated for use in western blotting, ICC & Immunofluorescence.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected Caspase-5 in P815 cells.
Immunofluorescence Analysis: 2 µg/mL from a representative lot detected Caspase-5 in P815 cells.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Quality
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Caspase-5 in Ramos cell lysate.
Target description
~48 kDa observed. Uncharacterized bands may appear in some lysates.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Ramos cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable. UniProt accession no.: P51878. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CASP5(838). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51204204
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- ABS445
- Temperature Control Device:
- Yes