ANTI-CASPASE 8 ANTIBODY MOUSE MONOCLONAL CLONE CAS8 PURIFIED FROM HYBRIDOMA CELL CULTURE

General description

Anti-Caspase 8 antibody, Mouse monoclonal (mouse IgM isotype) is derived from the CAS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human caspase 8. Caspase 8 gene is mapped to human chromosome 2q33.1 Caspases (Cysteine-requiring aspartate proteases) are a family of proteases. The caspase 8 is also known as MORT1-associated ced-3 homolog (MACH), FLICE (FADD-homologous ICE/ced-3-like protease) and Mch5. Eight isoforms of caspase 8 (caspase 8/a-h), including FLICE (CAP4/MACHα1), MACHα2 and MACHα3, MACHβ and Mch5 have been described at the mRNA level. Caspase 8 contains two amino-terminal tandem repeats within the prodomain, which are homologous to the death effector domain (DED) in the adaptor molecule Fas-associated death domain protein (FADD). It belongs to the tumor necrosis factor receptor family.

Specificity

Anti-Caspase 8 antibody, Mouse monoclonal reacts specifically with human caspase 8.

Immunogen

recombinant human caspase 8.

Application

Anti-Caspase 8 antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• western blotting
• immunocytochemistry

Monoclonal Anti-Caspase 8 is suitable for:

• indirect ELISA
• microarray
• western blot at a concentration of 5-10μg/mL using whole extract of a HeLa cells mitochondrial preparation

Biochem/physiol Actions

Caspase 8 (CASP8) undergoes a cleavage upon activation (leading to p26/p24/p18/p10 subunits, for isoforms 8/a and 8/b). Caspase-8 and caspase-10 are involved in death receptor-mediated apoptosis. CASP8 is a crucial defensive barrier against malignant proliferation and tumorigenesis by triggering the extrinsic apoptosis. Polymorphisms in the CASP8 gene is also an indicative biomarker for lung and breast cancers. Caspase 8 is also implicated in invasive colon cancer. Caspase 8 has been shown to process a variety of proapoptotic caspases, but it did not activate members of the ICE subfamily, except of ERICE (caspase 13). Monoclonal antibodies reacting specifically with caspase 8 are a useful tool for the study of the protease network involved in development and regulation, governing the life and death of cells and tissues.

Caspases (Cysteine-requiring Aspartate protease) are a family of proteases. Caspase-8 and -10 are involved in death receptor mediated apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.