Anti-Catechol O-methyltransferase/COMT Antibody (C15-1316-386)

General description

Catechol O-methyltransferase (EC 2.1.1.6; UniProt P21964) is encoded by the COMT gene (Gene ID 1312) in human. Catechol-O-methyltransferase initiates the metabolic breakdown of catecholamines (including the neurotransmitters dopamine, epinephrine, and norepinephrine) by catalyzing the transfer of a methyl group from S-adenosylmethionine (SAM) to catecholamines in the presence Mg++. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to endogenous substances, COMT is also important in the metabolism of catechol drugs. Two isoforms exist as a result of alternative initiation codon usage. The membrane-bound isoform or MB-COMT contains a short N-terminal cytoplasmic tail (a.a. 1-6), a transmembrane segment (a.a. 7-26), and a large C-terminal extracellular region (a.a. 27-271), while the soluble or S-COMT isoform lacks the N-terminal 50-amino acid sequence of MB-COMT. MB-COMT and S-COMT differ in their substrate affinity and capacities. Despite having a lower capacity than S-COMT, MB-COMT has around a 10-fold greater affinity for dopamine and noradrenaline, and therefore is likely to be better suited for metabolizing catecholamines at the concentrations found in brain. The influence of the Val(158)Met polymorphic form of catechol-o-methyltransferase (COMT) on brain activation and functional connectivity is the subject of numerous studies.

Specificity

This polyclonal antibody was raised against a sequence present in both the membrane-bound (MB-COMT) and the soluble (S-COMT) spliced isoforms of human catechol O-methyltransferase reported by UniProt (P21964).

Immunogen

Epitope: Extracellular domain.

KLH-conjugated linear peptide corresponding to an extracellular domain sequence of human catechol O-methyltransferase.

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Catechol O-methyltransferase/COMT in human prostate cancer, normal liver and kidney tissue sections.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-Catechol O-methyltransferase Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of COMT.

Quality

Evaluated by Western Blotting in human fetal liver tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Catechol O-methyltransferase/COMT in 10 µg of human fetal liver tissue lysate.

Target description

~28 kDa observed. 30.04 kDa (human MB-COMT) and 24.45 kDa (human S-COMT) calculated. Uncharacterized band(s) may appear in some lysates.

Linkage

Replaces: AB5873

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology), porcine (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_000745. UniProt accession no.: P21964. target post-translational modification: unmodified. Gene Information: human ... COMT(1312). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.