Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

General description

Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.

Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.

Immunogen

Epitope: within the heavy chain of mature cathepsin B

Human

purified human liver cathepsin B

Application

Immunoblotting (250 ng/ml)

Immunoprecipitation (1 µg/ml)

Immunofluorescence (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).

Reconstitution

Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.

Analysis Note

Positive Control
Hep G2 cells

Other Notes

Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.

This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany