Anti-Cav3.2 Calcium Channel Subunit Antibody, clone N55/10

General description

Voltage-gated calcium channel subunit alpha Cav3.2 is also called Voltage-dependent T-type calcium channel subunit alpha-1H and Low-voltage-activated calcium channel alpha 1 3.2 subunit. Voltage-gated calcium channel subunit alpha Cav3.2 and other voltage-sensitive calcium channels (VSCC) are involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Voltage-gated calcium channel subunit alpha Cav3.2 is expressed in kidney, liver, heart, brain, and isoform 2 appears to be testis specific. Voltage-gated calcium channel subunit alpha Cav3.2 defects can increase susceptibility to epilepsy, idiopathic generalized type 6 (EIG6) and epilepsy, childhood absence type 6 (ECA6).

Specificity

This antibody does not cross react with human Cav3.1 Calcium Channel Subunit (Prof. J. Trimmer, University of California, Davis.).

Immunogen

Recombinant protein corresponding to human Cav3.2 Calcium Channel Subunit.

Application

Detect Cav3.2 using this mouse monoclonal antibody, Anti-Cav3.2 Calcium Channel Subunit Antibody & clone N55/10 validated for use in western blotting.

Immunohistochemistry Analysis: A representative lot detected Cav3.2 Calcium Channel Subunit in wild type mice and demonstrated a loss of signal in Cav3.2 knock out mice (Prof. J. Trimmer, University of California, Davis.).

Western Blotting Analysis: A representative lot detected Cav3.2 Calcium Channel Subunit in HEK293 cell lysates expressing Flag-tagged Cav3.2 and demonstrated a loss of signal in HEK293 cell lysates expressing Flag-tagged Kv2.1 and Flag-tagged Cav3.1 (Prof. J. Trimmer, University of California, Davis.).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Cav3.2 Calcium Channel Subunit in 10 µg of NIH/3T3 cell lysate.

Target description

~260 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.