ANTI-CCL20/MIP-3A CLONE 1F5-H1 ZOOMAB« RABBIT MONOCLONAL (C005B-260556)

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F5-H1 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects C-C motif chemokine 20 (CCL20). It targets an epitope within 16 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human C-C motif chemokine 20 (CCL20).

Application

Quality Control Testing

Evaluated by Western Blotting using recombinant Human CCL20/MIP3 .

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant Human CCL20/MIP-3 .

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CCL20/MIP-3 in HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CCL20/MIP-3 in Human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human CCL20/MIP-3 protein with a KD of 1.3 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected CCL20/MIP-3 in CCL20/MIP-3 .

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

C-C motif chemokine 20 (UniProt: P78556; also known as Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Small-inducible cytokine A20) is encoded by the CCL20 (also known as LARC, MIP3A, SCYA20) gene (Gene ID: 6364) in human. CCL20 is a secreted protein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to produce the mature protein. The mature CCL20 can be further cleaved into 1-67, 1-64, and 2-70 fragments. It is expressed in a variety of human tissues and by a large number of immune cells. It is induced by bacterial lipopolysaccharides, TNFα, and Interferon-γ. It serves as a ligand for C-C-chemokine receptor 6 (CCR6). Its binding o CCR6 induces a strong chemotactic response and mobilization of intracellular calcium ions. CCL20-CCR6 pair is responsible for chemotaxis of dendritic cells, effector/memory T-cells and B-cells. CCL20 is reported to bind to a shallow extracellular pocket of CCR6 where the N-terminal tyrosine 27 and leucine 38 of CCR6 wrap onto the globular core of CCL20. It is shown to be highly active on resting memory T cells, indicating that it may be important for their recruitment to inflammatory sites early in an immune response. CCL20 is also involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation and is required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Wasilko, JD., et al. (2020). Nat. Commun. 11; Article 3031; Lee, AYS., et al. (2013). Front. Immunol. 4; 194; Schutyser, E., et al. (2000). J. Immunol. 165(8); 4470-4477).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.