Anti-CCM3/PDCD10 (C15-1317-322)

General description

Programmed cell death protein 10 (UniProt Q9BUL8; also known as Cerebral cavernous malformations 3 protein, TF-1 cell apoptosis-related protein 15) is encoded by the PDCD10 (also known as CCM3, TFAR15) gene (Gene ID 11235) in human. PDCD10/CCM3 is an ubiquitously expressed adaptor protein that plays a role in vascularization and vessel permeability, as well as in regulating cell survival and death. Both the N-terminal dimerization domain and the C-terminal focal adhesion targeting-homology (FAT-H) domain mediate its interaction with a diverse array of signaling partners. The FAT-H domain mediates PDCD10 interaction with CCM2 to form the KRIT1 CCM2 PDCD10 complex, as well as with striatin to form the striatin interacting phosphatase and kinase (STRIPAK) complex, while the dimerization domain mediates interactions with GCKIII serine/threonine kinases (MST4/MASK, STK24/MST3 and STK25). PDCD10 overexpression leads to increased cell migration, an essential process for blood vessel formation. Loss of either PDCD10 or STK24 increases neutrophil exocytosis as a result of disrupted interaction with UNC13D, a regulator of vesicle fusion. Loss-of-function mutations in CCM1/KRIT1, CCM2/OSM, or CCM3/PDCD10 gene cause cerebral cavernous malformations (CCMs).

Specificity

This polyclonal antibody detected the expected ~25 kDa target band in lysate from untreated, but not CCM3 shRNA-treated, EA.hy926 human umbilical vein endothelial cells (Courtesy of Kyle M. Draheim, Ph.D., Xiaofeng Li, Ph.D., Rong Zhang, David A.Calderwood, Ph.D. and Titus J. Boggon, Ph.D., Yale University, U.S.A.).

Immunogen

Recombinant full-length human CCM3/PDCD10.

Application

Anti-CCM3/PDCD10 Antibody, Cat. No. ABN1016, is a highly specific rabbit polyclonal antibody that targets CCM3/PDCD10 and has been tested in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:1,000 dilution from a representative detected 50 ng and 500 ng of recombinant human CCM3/PDCD10 (Courtesy of Kyle M. Draheim, Ph.D., Xiaofeng Li, Ph.D., Rong Zhang, David A.Calderwood, Ph.D. and Titus J. Boggon, Ph.D., Yale University, U.S.A.).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CCM3/PDCD10 in 100 µg of lysate from untreated, but not CCM3 shRNA-treated, EA.hy926 human umbilical vein endothelial cells (Courtesy of Kyle M. Draheim, Ph.D., Xiaofeng Li, Ph.D., Rong Zhang, David A.Calderwood, Ph.D. and Titus J. Boggon, Ph.D., Yale University, U.S.A.).

Quality

Evaluated by Western Blotting in EA.hy926 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected CCM3/PDCD10 in 10 µg of EA.hy926 human umbilical vein endothelial cell lysate.

Target description

~25 kDa observed. 24.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): Xenopus (based on high sequence homology), rat (based on high sequence homology), mouse (based on high sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_009148. UniProt accession no.: Q9BUL8. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... PDCD10(11235). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.