Anti-CD18 Mouse mAb (IB4)

General description

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.

Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).

Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.

Immunogen

Human

human peripheral blood monocytes

Application

Flow Cytometry (5-10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Cell Inhibition Assays (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Analysis Note

Negative Control
Nalm-6 cells

Positive Control
HPB-MLT cells

Other Notes

Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.

Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany