ANTI-CD28 CLONE 37.51

General description

T-cell-specific surface glycoprotein CD28 (UniProt: P31041, also known as CD28) is encoded by the Cd28 gene (Gene ID: 12487) in murine species. CD28 is a single-pass type I membrane protein that exists as a disulfide linked homodimer. In human CD28 is expressed by a majority of T cells in the periphery, including all CD4+ cells and about 50% CD8+ cells. CD28 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 2-150), a transmembrane domain (aa 151-177), and a cytoplasmic domain (aa 178-218). CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. It enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. B7-1 (CD80) and B7-2 (CD86) are two known ligands of CD28 on activated B cells and antigen presenting cells (APC). These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction co-stimulatory signaling between the B7 ligands and CD28 is essential for crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Specificity

Clone 37.51 is a Syrian hamster monoclonal antibody that detects CD28 in murine cells.

Immunogen

EL-4 T lymphoma cells.

Application

Affects Function Analysis: A representative lot detected CD28 in Affects Function applications (Ulges, A., et. al. (2015). Nat Immunol. 16(3):267-75; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65; Brender, C., et. al. (2007) Blood. 1;110(7):2528-36).

Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65).

Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8).

Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8

This syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28.

Quality

Evaluated by Flow Cytometry in EL-4 T lymphoma cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells.

Target description

25.24 kDa calculated.

Physical form

Format: Purified

Purified syrian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.

Other Notes

Concentration: Please refer to lot specific datasheet.