Specificity
The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.
KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.
FUSION PARTNER: NSO myeloma cell line
Immunogen
Human endothelial vesicles
Application
Anti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).
Identification of haemopoietic progenitor cells
Studies into the control mechanisms of early haemopoiesis
Purification of haemopoietic cells from, for example, FACS
The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts
Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.
Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Quality
Tested
Physical form
Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage and Stability
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Other Notes
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CBL496
