Anti-CD54 Mouse mAb (15.2)

General description

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Protein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).

Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.

Immunogen

Human

human monocytes

Application

Flow Cytometry (1-10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Analysis Note

Positive Control
Raji cells

Other Notes

FACS was performed on 5x10⁵ cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell68, 71.
Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany