ANTI-CD63 CLONE 3H6 ZOOMAB RB MONO (C005B-261159)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3H6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CD63 (TSpan30/LAMP3). It targets an epitope within 22 amino acids from the second extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the second extracellular domain of human CD63.

Application

Quality Control Testing

Evaluated by Western Blotting in Human Platelets tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD63 in Human Platelets tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD63 in human bone marrow tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CD63 peptide with a KD value of 8.6 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CD63, clone 3H6 ZooMAb^®, Cat. No. ZRB1864, is a recombinant Rabbit monoclonal antibody that specifically targets CD63 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human. CD63 is a multi-pass membrane glycoprotein of the tetraspanin family that has three cytoplasmic domains, four transmembrane domains, and two extracellular domains. CD63 is detected in endosomes, lysosomes, and plasma membranes. It has also been detected in platelets, radial growth phase primary melanomas, hematopoietic cells, and in tissue macrophages. In melanoma cells it is involved in their motility and adhesion. It plays a role in the adhesion of leukocytes onto endothelial cells and is involved in the activation of ITGB1 and integrin signaling that leads to the activation of AKT, FAK/PTK2 and MAP kinases and promote cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration. CD63 bears a YXXØ consensus motif in its carboxy terminal cytoplasmic domain with an essential tyrosine residue, 2 hydrophobic XX residues, and a bulky hydrophobic amino acid. This motif is required for endocytosis at the plasma membrane. Lack of expression of CD63 in platelets has been reported in Hermansky-Pudlak syndrome that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. Overexpression of CD63 has been observed in esophageal cancer that is negatively correlated with tumor stage and lymph node metastasis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pols, MS., and Klumperman, J. (2009). Exp. Cell Res. 315(9); 1584-1592).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.