ANTI-CD84/SLAMF5 CLONE 1D3

General description

SLAM family member 5 (UniProt Q18PI6; also known as Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5, CD84) is encoded by the Cd84 (also known as Slamf5) gene (Gene ID 12523) in murine species. CD84 belongs to the 7-member (SLAM/CD150, CD48/SLAMF2, Ly-9/CD229, 2B4/CD244, CD84/SLAMF5, Ly108/NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with eath other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. SLAMF members carry one or more copies of immunoreceptor tyrosine-based switch motif or ITSM (TxYxxV/I) in their cytoplasmic tails. Upon phosphorylation on the tyrosine residue, ITSM mediates the recruitment of SH2 domain-containing signaling molecules such as SLAM-associated protein (SAP), including SAP in T-, NK, and NK T-cells, EAT-2A and EAT-2B (murine) in NK cells and APCs. Murine CD84 is produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature CD84 with an extracellular region (a.a. 22-221) that contains a V-type Ig-like domain (a.a. 26-129) and a C2-type Ig-like domain (a.a. 132-206), followed by a single transmembrane (a.a. 222-242) and a cytoplasmic tail (a.a. 243-329) with two copies of ITSM (a.a. 263-268 & 298-303). Alternative splicings produce three CD84 isoforms, with spliced isoform 2 produced as a truncated extracellular domain with an alternative C-terminal sequence (from 128-RLKTPKITQSLIS-140 to 128-KLWQHGALDLLLI-140) and no transmembrane or cytoplasmic sequence (a.a. 141-329 missing).

Specificity

Clone 1D3 immunostained the surface of murine CD84-transfected, but not untranfected, BI-141 murine T-cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305). Reactivity toward spliced isoform 2 has not been determined.

Immunogen

Epitope: extracellular domain

Human IgG1 Fc-tagged recombinant mouse CD84 extracellular domain.

Application

Flow Cytometry Analysis: 0.5 µg from a representative lot detected CD84-positive cells among 2 million murine splenocytes (Courtesy of Dr. André Veillette, IRCM, Montréal, Canada).

Flow Cytometry Analysis: A representative lot detected a reduced CD84 expression on the surface of NK cells from CD45-knockout mice when compared with NK cells from wild-type mice (Guo, H., et al. (2015). Mol. Cell. Biol. 35(1):41-51).

Flow Cytometry Analysis: A representative lot detected endogenous CD84 on the surface of DX5+CD3 murine splenic NK cells, as well as the exogenously expressed murine CD84 on the surface of transfected BI-141 murine T-cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).

Research Category
Inflammation & Immunology

This rat monoclonal Anti-CD84/Slamf5, clone 1D3 Antibody, Cat. No. MABF929 is validated for use in Flow Cytometry for the detection of CD84/Slamf5.

Quality

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG1 .

Target description

34.85/37.38 (mature/pro-isoform 1), 13.64/16.17 (mature/pro-isoform 2), 34.78/37.31 (mature/pro-isoform 3) calculted.

Physical form

Format: Purified

Protein G purified.

Purified rat IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.