Anti-Cdc4 Antibody (C15-1315-672)

General description

The F-box protein family is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. They constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
Cdc4 is one member of this family, which is also known as AGO, FBW7, FBX30 or SEL10. CDC4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Cdc4 proteins of amino acid lengths varying from 553 aa to 707 aa in length have been reported. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event.5,6 Cdc4 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. Thus, it may be important for Alzheimer′s disease. Mutations of the CDC4 gene are detected in ovarian and breast cancer cell lines and the CDC4 gene may also be involved in the pathogenesis of human pancreatic and endometrial cancers.

Specificity

Reactivity has been confirmed with human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.

Recognizes CDC4.

Immunogen

Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10).

Application

Anti-Cdc4 Antibody is an antibody against Cdc4 for use in WB & IP.

Research Category
Protein Trafficking

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Western Blotting: 1-3 μg/mL
Immunoprecipitation: 7 µg/reaction
Optimal working dilutions must be determined by end user.

Physical form

ImmunoAffinity Purified

This polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.

Storage and Stability

Store at -20°C in undiluted aliquots for 1 year from date of shipment.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_001013415.1, NM_018315.4, NM_033632.2. UniProt accession no.: Q969H0. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... FBXW7(55294). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.