ANTI-CDCP1 ANTIBODY (C005B-254268)

General description

Neurexin-1 (UniProt: Q9CS84; also known as Neurexin I-alpha, Neurexin-1-alpha) is encoded by the Nrxn1 (also known as Kiaa0578) gene (Gene ID: 18189) in murine species. Neurexins are a large family of proteins that act as neuronal cell-surface receptors. Their function appears to be isoform-specific. They are heavily glycosylated and processed neuronal transmembrane cell surface proteins that are involved in cell recognition and adhesion. Neurexin-1 (the isoform) is a single-pass type I presynaptic membrane glycoprotein that is synthesized with a signal peptide that is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 31-1438), a transmembrane domain (aa 1439-2459), and a cytoplasmic domain (aa 1460-1514). It has a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. It is involved in the regulation of calcium channel activity and Ca2+-triggered neurotransmitter release at synapses and at neuromuscular junctions. It binds to neuroligins through an extracellular laminin neurexin sex-hormone-binding protein (LNS) domain. The neuroligin neurexin complex, a heterophilic adhesion system, promotes assembly and maturation of synapses through bidirectional signaling. In this complex, postsynaptic neuroligins interact trans-synaptically with presynaptic neurexins. This pan-neurexin antibody recognizes - and β-neurexins. Seven isoforms of Neurexin-1 have been described that are produced by alternate promoter usage and alternative splicing. (Ref.: Taniguchi, H., et al. (2007). J. Neurosci. 27(11); 2815-2824; Sons, MS., et al. (2006). Neuroscience. 138(2); 433-446; Missler, M., et al. (2003). Nature. 423(6943); 939-948; Dean, C., et al. (2003). Nat. Neurosci. 6(7); 708-716).

Specificity

This chicken polyclonal antibody detects Neurexin-1. It targets an epitope within the C-terminal, cytoplasmic domain and is predicted to react with all isoforms.

Immunogen

GST-tagged recombinant fragment corresponding to 51 amino acids from the C-terminal, cytoplasmic domain of human Neurexin-1.

Application

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neurexin-1 in Human brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neurexin-1 in lysates from HEK293 cells and Mouse brain tissue.

Electron Microscopy: A representative lot detected Neurexin-1 in Electron Microscopy applications (Taniguchi, H. et al. (2007). J Neurosci. 27(11): 2815-2824).

Immunofluorescence Analysis: A representative lot detected Neurexin-1 in Immunofluorescence applications (Dean, C. et al. (2003). Nat Neurosci. 6, 708-716).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Physical form

Purified chicken polyclonal antibody in buffer containing 50 mM HEPES pH 7.4, 150 mM NaCl, 20% Glycerol with 0.02% sodium azide with 20% glycerol.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.