ANTI-CDK1/CDC2 (PSTAIR) ANTIBODY

General description

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.

Specificity

Cdk1/cdc2 kinase

Wide species cross-reactivity is expected.

Immunogen

Synthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species.

Application

Fluorescent Immunocytochemistry Analysis:
A previous lot of this antibody was used in the confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor488), and DAPI (Blue, Nucleus). Positive nuclear staining.

Immunoprecipitation: 4 µg of a previous lot immunoprecipitated cdc2 from 500 µg of A431 RIPA lysate.

Anti-Cdk1/Cdc2 (PSTAIR) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Cdk1/Cdc2 (PSTAIR) & has been validated in ICC, IP & WB.

Quality

Evaluated by western blot on human A431, mouse 3T3 and/or rat L6 RIPA cell lysates.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected cdc2 from human A431, mouse 3T3 or rat L6 RIPA cell lysates.

Target description

~34 kDa

Physical form

Format: Purified

Purified rabbit serum in Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.

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