General description

The carcinoembryonic antigen- (CEA-) related cell adhesion molecules (CEACAMs) constitute a 12-member subgroup of the CEA family of immunoglobulin-related proteins first described in 1965 (PMID 4953873). CEACAMs are reported to participate in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEACAM molecules from neighbouring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-cell adhesion through trans-oligomerization. CEACAM molecules within the same cell can also undergo transmembrane domain-mediated cis-oligomerization, an event important for sustaining downstream cellular signaling. Athough CEACAM1 is shown to utilize its cytoplasmic domain for transducing cellular signaling, not all CEACAM members are expressed with a significant cytoplasmic domain, and others (CEACAM5/6/7/8) are GPI-anchored without even a transmembrane domain. (PMID 4735478). Human CEACAM6 (also known as CD66c, Non-specific crossreacting antigen, Normal cross-reacting antigen; UniProt P40199) is encoded by the CEACAM6 (or CEAL & NCA) gene (NCBI Gene ID 4680). It is a GPI-anchored CEACAM with three extracellular Ig domains. Clone 1H7-4B does not cross-react with human CEACAM1/3/4/5/7/8, rat CECAM1, or mouse CEACAM1/2.

Specificity

Clone 1H7-4B specifically detected exogenously expressed human CEACAM6, but not CEACAM1/3/4/5/7/8, on the surface of transfected CHO cells.

Immunogen

Epitope: Extracellular domain.

Recombinant human CEACAM6 extracellular domain.

Application

Anti-CEACAM6 Antibody, clone 1H7-4B is an antibody against CEACAM6 for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA, Immunoprecipitation, Immunocytochemistry, Flow Cytometry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CEACAM6 in normal (colon, lung, lymph node) and cancer (lung, colon) human tissue sections.

Flow Cytometry Analysis: A representative lot detected exogenously expressed human CEACAM6 on the surface of transfected CHO cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

ELISA Analysis: Representative lots detected CEACAM6 in multivesicular bodies (MVBs) from 2 day-starved HT29 human epithelial cells and T102/3 human colon epithelial cancer cells, as well as in human bronchoalveolar lavage fluid (BALF) samples (Singer, B.B., et al. (2014). PLoS One. 9(4):e94106; Klaile, E., et al. (2013). Respir. Res. 14:85).

Immunohistochemistry Analysis: A representative lot detected CEACAM6 in paraffin-embedded human lung cancer tissue sections (Klaile, E., et al. (2013) Respir Res. 14:85).

Western Blotting Analysis: A representative lot detected CEACAM6 in 2 day-starved HT29 human epithelial cells and HT29-derived multivesicular bodies (MVBs) (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CEACAM6 in 10 µg of HT-29 cell lysate.

Target description

~90 kDa observed. Target band size appears larger than the calculated molecular weights of 33.48/37.20 kDa (mature/pro-form) due to high glycosylation.

Physical form

Format: Purified

Protein G Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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