Anti-CENP-A antibody produced in rabbit

General description

CENP-A is a 17 kDa histone H3- like protein.

Centromere protein A is a protein encoded by the CENPA gene in humans. It is referred as CenH3 and CENP-A. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on a chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.

Immunogen

synthetic peptide corresponding to amino acids 32-49 located near the N-terminus of human CENP-A, conjugated to KLH.

Application

Anti-CENP-A antibody has been used in immunoblotting.

Anti-CENP-A antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using HEK-293 cells expressing human CENP-A.

Biochem/physiol Actions

The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of (CENP-A) plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.