Anti-Centrin antibody produced in rabbit

General description

Centrins are acidic proteins, that are usually found in all eukaryotes. Centrin 1 (CETN1), an intron-less gene is mapped on human chromosome 18p11. It possesses certain features of an expressed retroposon.

The centrosome, the major microtubule-organizing center (MTOC) in the animal cell, is a key determinant in the organization of the cytoplasm, in the determination of cell polarity, and in the establishment of mitotic spindle poles during the cell cycle. Centrin is a member of the calcium binding E-F hand protein superfamily present in the centrosome pericentriolar matrix.
Three separate centrin genes have been identified in human cells: centrin 1, 2, and 3. Centrin 1 shows strong amino acid homology to centrin 2 (85%), but lower homology to centrin 3 (54%) and CDC31 (50%) from the yeast S. cerevisiae. Centrin has a fundamental role in the MTOC structure and function. Phenotypic analysis of mutants in Chlamydomonas caltractin and the yeast CDC31 genes have indicated that the protein is required for proper duplication and segregation of the MTOC in the respective cells.

Immunogen

synthetic peptide corresponding to the C-terminus of human centrin 1 (amino acids 157-172). The sequence is highly conserved in centrin 1 of many species, e.g. mouse, Drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3.

Application

Anti-Centrin antibody produced in rabbit has been used in:

• immunofluorescence
• immunoblotting
• immunofluorescence microscopy

Biochem/physiol Actions

Centrin has a fundamental role in the microtubule-organizing center (MTOC) structure and function. These proteins has imperative roles in centrosome positioning and segregation. Centrin also participates in cytokinesis and cell cycle progression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.