Anti-CEP152 Antibody, N-Term (C15-1316-972)

General description

Centrosomal protein of 152 kDa (UniProt O94986; also known as Cep152) is encoded by the CEP152 (also known as KIAA0912) gene (Gene ID 22995) in human. The mammalian Asl (Asterless) ortholog Cep152 is a centrosomal protein (CEP) that plays an important role in centriole duplication and centriole-to-centrosome conversion. The centrosome is the main microtubule-organizing center essential spindle formation and chromosome segregation during mitosis. Centrosomes are composed of two orthogonally arranged centrioles surrounded by pericentriolar material (PCM). Centriole duplication begins by assembling a procentriole in G1/S phase, where centrosomal scaffolds Cep152 and Cep192 (Spd-2 ortholog) mediate the recruitment of polo kinase Plk4 to centrosomes. This is a key event that triggers the entire duplication process. New centrioles assemble perpendicularly to pre-existing ones in G1/S phase and elongate throughout S and G2 phase. The conversion of fully elongated daughter centrioles into centrosomes during mitotic progression requires sequential loading of Cep135, Cep295 (Ana1 analog) and Cep152 onto daughter centrioles.Cep152 is also an important regulator of genomic integrity and cellular response to DNA damage. Overexpression of CEP152 can drive amplification of centrioles. CEP152 gene mutations are identified among individuals with Seckel syndrome, a heterogeneous autosomal recessive disorder characterized by a proportionate short stature, severe microcephaly and mental retardation, and a typical ′bird-head′ facial appearance. Cep152 impairment in Seckel patients-derived cells leads to accumulation of genomic defects as a result of replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.

Specificity

This polyclonal antibody specifically detects human CEP152 protein. It targets an epitope within the first 87 amino acids from the N-terminal region. Recognizes both long and short isoforms of Cep152.

Immunogen

Epitope: N-terminus

His-tagged recombinat fragment corresponding to 87 amino acids from the N-terminal end of human Centrosomal protein of 152 kDa (CEP152).

Application

Anti-CEP152, N-Term, Cat. No. ABE1856, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 152KDa and has been tested in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Electron Microscopy Analysis: A representative lot detected CEP152 in U2OS cells (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33).

Western Blotting Analysis: A representative lot detected CEP152 in U2OS cells transfected with myc-Plk4 and HEK293T cells transfected with Flag-Cep152-2 (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33).

Immunocytochemistry Analysis: A representative lot detected CEP152, N-Term in U2OS cells (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33;Greitzer-Antes, D., et. al. (2013). J cell Sci. 126(Pt 13):2914-23).

Immunoprecipitation Analysis: A representative lot detected CEP152, N-Term in HEK293T cells transfected with Flag-Cep152-2 (Sonnen, K.F., et. al. (2013). J cell Sci. 126(Pt 14):3223-33).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CEP152 in 10 µg of U2OS cell lysate.

Target description

~230 kDa observed; 195.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. technique(s): electron microscopy: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_055800. UniProt accession no.: O94986. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... CEP152(22995). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.