ANTI-CEP164 (C005B-240877)

General description

Centrosomal protein of 164 kDa (UniProt: Q9UPV0; also known as Cep164) is encoded by the CEP164 (also known as KIAA1052, NPHP15) gene (Gene ID: 22897) in human. Cep164 is expressed in several cell lines and it plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Cep164 localizes specifically to very distally located appendage structures on the mature centriole from which PC formation originates and it persists at centrioles throughout mitosis. Hence, it can serve as a marker for distal appendages on mature centrioles or basal bodies. Cep164 also plays a vital role in G2/M checkpoint and nuclear divisions. It is a key participant in the DNA damage-activated ATR/ATM signaling cascade and is required for the proper phosphorylation of H2AX, replication protein A, and check point kinases 1 and 2. Its phosphorylation at Ser186 is induced upon DNA-damage caused by treatment with IR irradiation, UV irradiation, hydroxyurea or amphidicolin. Mutations in CEP164 gene are known to cause nephronophthisis, a disease characterized by blindness in childhood, facial dysmorphism, bronchieclasis, liver failure and progression to end-stage renal failure.

Specificity

This polyclonal antibody detects Centrosomal protein of 164 kDa in human. It targets an epitope within the first 298 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus

His-taggged recombinant fragment of the first 298 amino acids from human Centrosomal protein of 164 kDa (CEP164).

Application

Anti-CEP164, Cat. No. ABE2621, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 164 kDa and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected CEP164 in U2OS cells and hTERT-RPE1 cells that were serum starved for 48 hours (Graser, S., et. al. (2007). J Cell Biol. 179(2):321-30; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).

Western Blotting Analysis: A representative lot detected CEP164 in total lysates of HeLa S3, U2OS, and 293T cells as well as 293T cells overexpressing myc-Cep164 (Graser, S., et. al. (2007). J Cell Biol. 179(2):321-30).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CEP164 in 10 µg of U2OS cell lysate.

Target description

~200 kDa observed; 164.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. technique(s): immunocytochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_055771. UniProt accession no.: Q9UPV0. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... CEP164(22897). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.