ANTI-CEREBLON CLONE 4A7H6

General description

Protein cereblon (UniProt Q96SW2) is encoded by the CRBN (also known as AD-006, MRT2A) gene (Gene ID 51185) in human. Originally identified as a genetic cause of inherited autosomal recessive mental retardation, the human CRBN gene encodes a substrate receptor of a ubiquitin E3 cullin 4-RING ligase (CRL4) complex. A cullin-4 (CULT4) protein provides a scaffold for assembling RING box-domain protein (​RBX1) and the adaptor protein damaged ​DNA-binding protein 1 (​DDB1) to form a CRL4 E3 complex, where ​RBX1 serves as the docking site for the activated E2 protein, and ​DDB1 recruits substrate-specificity receptors or DCAFs (DDB1-cullin 4-associated factors) to form the substrate-presenting side of the CRL4 complex. Thalidomide and its analogs (lenalidomide, pomalidomide and apremilast) are immunomodulatory drugs (IMiDs) that exert their antiproliferative effects on multiple myeloma (MM) cells and immunomodulatory effects on T cells by directly binding cereblon and promoting recruitment of the hematopoietic zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), leading to their ubiquitination and proteasomal degradation. Human cereblon is a 442-amino acid protein composed of a ​Lon protease-like scaffold (a.a. 80-317) with a helical insertion that mediates ​DDB1 binding and a thalidomide-binding domain (TBD; a.a. 339-442).

Specificity

Clone 4A7H6 was raised against a sequence N-terminal to the LON domain present in all spliced isoforms of bovine, chicken, human, mouse, orangutan, rat, and xenopus (UniProt Q0P564, P0CF65, Q96SW2, Q8C7D2, Q5R6Y2, Q56AP7, Q640S2). Immunogen sequence is well conserved in essentially all mammalian species.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence N-terminal to the LON domain of cereblon.

Application

Detect Cereblon using this mouse monoclonal antibody, Anti-Cereblon, clone 4A7H6, Cat. No. MABN1849, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected cereblon in human cerebral cortex, kidney, and tonsil tissue sections.

Western Blotting Analysis: A 1:125 dilution from a representative lot detected cereblon in 10 µg of HEK293 cell lysate.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected cereblon in 10 µg of Jurkat cell lysate.

Target description

~50 kDa observed. 50.55/50.48 kDa (human isoform 1/2), 50.88/49.48/50.81 kDa (mouse isoform 1/2/3), 50.80/51.12/49.25/50.88/50.67 kDa (bovine/chicken/orangutan/rat/xenopus) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.