ANTI-CHIP RABBIT PAB

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.

Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.

This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.

Immunogen

a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence

Application

Immunoblotting (1:200-1:1000)

Immunofluorescence (1:250)

Immunoprecipitation (1:20)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
COS-7 cells

Other Notes

May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.

Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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