ANTI-CHLAMYDIAL MOMP CLONE B-D3 (C005B-450515)

General description

Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV). Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants. These are identified based on serological reactivity of the epitopes on their outer membrane. Intracellularly chlamydia replicates within a vacuole. Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles. This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell. Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate. Clone B-D3 specifically detects surface exposed epitopes on major outer membrane protein (MOMP) from Chlamydia genus. It displays strong reactivity with Chlamydial serovars B, Ba, D, E, and L2 and weak reactivity with L1. MOMP, a cysteine-rich molecule, plays a vital role in maintaining the structural and functional properties of the chlamydial outer membrane. It contains determinants that confer type, subspecies, and species antigenic properties to the protein. It displays high sensitivity to heat and trypsin treatment that destroys MOMP epitopes recognized by this clone. (Ref.: Zhang, YX et al., (1987). J. Immunol. 138(2); 575-581).

Specificity

Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.

Immunogen

Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).

Application

Anti-chlamydial MOMP, clone B-D3, Cat. No. MABF2129, is a mouse monoclonal antibody that detects Chlamydial major outer membrane protein (MOMP) and has been tested for use in Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Neutralizing applications.

Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).

Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).

Research Category
Inflammation & Immunology

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.

Target description

~43 kDa observed.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.