General description
Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Specificity
Recognizes cholinergic neurons in brain and central nervous systems.
Immunogen
Human placental enzyme.
Application
Anti-Choline Acetyltransferase Antibody is an antibody against Choline Acetyltransferase for use in IH & WB.
Immunoblot: 1:1,000-1:5,000
Immunohistochemistry: 1:500-1:1,000. See page two of this data sheet.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Target description
70 kDa
Physical form
Goat antisera, liquid, no preservatives.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Rat brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: goat. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: guinea pig, mouse, rat. manufacturer/tradename: Chemicon®. . technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_020549.3, NM_020984.2, NM_020985.2, NM_020986.2. UniProt accession no.: P28329. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: mouse ... Chat(12647)rat ... Chat(290567). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51151500
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- AB144
- Temperature Control Device:
- Yes
