Specificity
Reacts with Choline Acetyltransferase (ChAT).
Immunogen
Purified human placenta ChAT.
Application
Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.
Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.
ELISA (two side)
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified immunoglobulin. Liquid.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51151500
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB5350
- Temperature Control Device:
- Yes
