ANTI-CHROMOBOX PROTEIN HOMOLOG 3 (RABBIT POLYCLONAL) (C15-1316-885)

General description

Chromobox protein homolog 3, also known as Chromobox protein homolog 3 (HECH), Heterochromatin protein 1 homolog gamma (HP1 gamma), or Modifier 2 protein, and encoded by the gene name CBX3, is implied to be involved in transcriptional silencing in heterochromatin-like complexes. Chromobox protein homolog 3 recognizes and binds histone H3 tails methylated at ′Lys-9′, leading to epigenetic repression. Chromobox protein homolog 3 may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Chromobox protein homolog 3 may also play a role in the formation of functional kinetochore through interaction with MIS12 complex proteins. Chromobox protein homolog 3 binds directly to CHAF1A and it has been shown to interact with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2, SP100, MIS12, DSN1, CBX5 via the chromoshadow domain, POGZ, CHAMP1, and ASXL1. Chromobox protein homolog 3 is phosphorylated by PIM1. Recent studies have implied that Chromobox protein homolog 3 is phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

Immunogen

Epitope: Chromo Domain 1

KLH-conjugated linear peptide corresponding to Chromo Domain 1 of human Chromobox protein homolog 3.

Application

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Chromobox protein homolog 3 in 500 µg of HeLa nuclear extract.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Chromobox protein homolog 3 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Chromobox protein homolog 3 antibody is validated for use in WB, IC;IP for the detection of Chromobox protein homolog 3.

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Chromobox protein homolog 3 in 10 µg of HeLa nuclear extract.

Target description

~22 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), horse (based on 100% sequence homology), monkey (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_009207. UniProt accession no.: Q13185. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CBX3(11335). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.