ANTI-CLAUDIN-4 ANTIBODY CLONE 10B4.3 (C15-1885-105)

General description

Alpha-Parvin (UniProt: Q9NVD7; also known as Actopaxin, CH-ILKBP, Calponin-like integrin-linked kinase-binding protein, Matrix-remodeling-associated protein 2) is encoded by the PARVA (also known as MXRA2) gene (Gene ID: 55742) in human. a-Parvin is a widely expressed, highly conserved, peripheral membrane protein that is highly expressed in heart, skeletal muscle, kidney, and liver. It contains two calponin-homology (CH1 and CH2) domains that are localized in amino acids 95-202 and 262-369. It interacts with the carboxy terminal of integrin-linked kinase (ILK) via its CH2 domain and deletion of its CH2 domain is reported to abolish its ability to localize to focal adhesions. a-Parvin plays a role in sarcomere organization and in smooth muscle cell contraction and is also required for normal development of the embryonic cardiovascular system. It is also essential for normal septation of the heart outflow tract. a-Parvin also plays a role in sprouting angiogenesis and is required for normal adhesion of vascular smooth muscle cells to endothelial cells during blood vessel development. It is also reported to play a role in establishing cell polarity, reorganization of the actin cytoskeleton, formation of lamellipodia and ciliogenesis. (Ref.: Tu, Y., et al. (2001). J. Cell Biol. 153(3); 585-598).

Specificity

Clone 1D4 is a mouse monoclonal antibody that detects a-Parvin. It targets an epitope within 29 amino acids from the C-terminal half.

Immunogen

GST-tagged recombinant fragment corresponding to 351 amino acids from human a-Parvin.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in C2C12 cell line.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected a-Parvin in C2C12 cell line.

Tested Applications

Western Blotting Analysis: A representative lot detected a-Parvin in Western Blotting applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

Immunofluorescence Analysis: A representative lot detected a-Parvin in Immunofluorescsence applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

ELISA Analysis: A representative lot detected a-Parvin in ELISA applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

Immunoprecipitation Analysis: A representative lot detected a-Parvin in Immunoprecipitation applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-a-Parvin, clone 1D4 , Cat. No. MABT1441, is a mouse monoclonal antibody that detects a-Parvin and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.