ANTI-CLAUDIN-4 ANTIBODY CLONE 10B4.3 (C15-1885-108)

Specificity

Clone FIB504 is a rat monoclonal antibody that detects Integrin β7. It targets an epitope within the D2 region in the extracellular domain.

Immunogen

TK-1 murine T lymphoblast cells.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected Integrin β7 in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Inhibition: A representative lot inhibited adhesion of TK1 cells to VCAM-1, Fibronectin, and MAdCAM-1.(Andrew, D.P., et al. (1994). J Immunol. 153(9):3847-61).

Flow Cytometry Analysis: A representative lot detected Integrin β7 in Flow Cytometry application. (Rott, L.S., et al. (1997). J Clin Invest.100(5):1204-8; Ballet, R., et al. (2021). Nat Immunol.;22(3):381-390).

Immunohistochemistry Applications: A representative lot detected Integrin β7 in Immunohistochemistry applications (Dearling, J.L.J. et al. (2016). Inflamm Bowel Dis. 22(3)529-38).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Integrin beta-7 (UniProt: P26011; also known as Integrin beta-P, M290 IEL antigen) is encoded by the Itbg7 gene (Gene ID: 16421) in murine species. Integrins are heterodimeric integral membrane proteins composed of a variable -subunit and a conserved β-subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various and β-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the -subunit. Integrin β7 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form that contains a large cytoplasmic domain (aa 20-724), a transmembrane domain (aa 725-745), and a cytoplasmic domain (aa 746-806). It associates with associates with either 4 or E (ITGAE). Eβ7 can then function as a receptor for E-cadherin. 4β7 is involved in adhesive interactions of leukocytes and serves as a receptor for fibronectin. It recognizes one more domains within the alternatively spliced CS-1 region of fibronectin. It can also serve as receptor for MAdCAM1 and VCAM1 and is reported to recognize the sequence L-D-T in MAdCAM1. Shp1 is shown to selectively inhibit endocytosis of β7, which enhances surface 4β7 display and lymphocyte homing to gut-associated lymphoid tissue. Shp1 is reported to restrain plasma membrane β7 phosphorylation and inhibit β7 endocytosis. Clone FIB504 is reported to functionally block human 4β7. (Ref.: Ballet, R., et al. (2021). Nat. Immunol. 22(3); 381-390; Andrew, DP., et al. (1994). J. Immunol. 153(9); 3847-3861).

Physical form

Purified rat monoclonal antibody IgG2a in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.