General description
Calcium-activated chloride channel regulator 4 (UniProt Q14CN2; also known as CaCC-2, Calcium-activated chloride channel family member 4, Calcium-activated chloride channel protein 2, hCaCC-2, hCLCA4) is encoded by the CLCA4 (also known as CaCC2) gene (ORF UNQ562/PRO1124; Gene ID 22802) in human. CLCA4 (CaCC2) belongs to the calcium-activated chloride channel regulator (previously known as chloride channel calcium activated or CLCA) family of self-cleaving, secreted metalloproteases that activate calcium-dependent chloride currents (ICaCC) in mammalian cells. Originally thought to be calcium-activated chloride channels (CaCCs) themselves, CLCA proteins were later identified as secreted CaCCs modulators. Among the four CLCA genes identified in human genome, only three (CLCA1/CACC1, CLCA2/CACC3, CLCA4/CACC2) encode functional Cl channel accessory proteins. CLCA4 is predominantly expressed in colon along with CLCA1, and both are reportedly downregulated in colorectal cancer cells. CLCA4 is also reported to be downregulated in breast cancer cells and exogenous CLCA4 overexpression inhibits breast cancer cell proliferation, while CLCA4 knockdown induces epithelial-to-mesenchymal transition (EMT) in mammary epithelial cells. CLCA4 is produced with a signal peptide sequence (a.a. 1-21) that is removed posttranslationally. Similar to other CLCA members, CLCA4 is further processed proteolytically by its metalloprotease domain (a.a. 45-119) to yield a 110 kDa soluble form and a 30 kDa membrane-bound form. The soluble fragment of all CLCA members also contains a von Willebrand factor type A (VWA) domain that in the case of CLCA2 is shown to engage interaction with beta4-integrin.
Specificity
Clone 1C5.1 recognizes a metalloprotease domain epitope that is not present in the 30 kDa membrane-bound form or the spliced isoforms 2 reported by UniProt (Q14CN2).
Immunogen
Epitope: Metalloprotease domain.
GST-tagged recombinant human CLCA4 metalloprotease domain fragment (~40 kDa).
Application
Anti-CLCA4 Antibody, clone 1C5.1 is an antibody against CLCA4 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CLCA4 in human cerebellum, colon, kidney and testis tissue sections.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Quality
Evaluated by Western Blotting of human CLCA4 GST fusion protein.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human CLCA4 metalloprotease domain fragment GST fusion protein.
Target description
101.3 kDa (pro-form) and 98.89 kDa (mature form) calculated. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 51284019
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABN1581
- Temperature Control Device:
- Yes
