Anti-CLDN1 Mouse mAb (1C5-D9)

General description

Purified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.

Recognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.

This Anti-CLDN1 Mouse mAb (1C5-D9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of CLDN1.

Immunogen

Full-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3-5 &microg/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
PRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Guevin C, et al. 2009. Antiviral Res.84, 14.
Sainz, B., Jr, et al. 2009. Virol. J.6, 103.
Stamataki Z, et al. 2009. Blood113 585.
Farquhar MJ, et al. 2008. J. Virol.82 8797.

Legal Information

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