Anti-CLIM-1/2 Antibody (C15-1316-999)

General description

LIM domain-binding protein 1 (UniProt P70662; CLIM-2, LDB-1) encoded by the murine Ldb2 gene (Clim2, Nli; Gene ID 16825) and LIM domain-binding protein 2 (UniProt O55203; CLIM-1, LDB-2, CLP-36) encoded by the murine Ldb2 gene (Clim1; Gene ID 16826) are LIM domain-interacting cofactors whose interaction with LIM homeodomain (LIM-HD) transcription factors are crucial for the development of specific types of interneurons and motor neurons during neural tube development. The ubiquitin ligase RLIM targets CLIM for proteasomal degradation, thereby inhibiting developmental LIM-HD activity.

Specificity

Reacts with both CLIM1 and CLIM2. Expected to react with all spliced isoforms of CLIM1 (CLIM-1a, CLIM-1b, and isoform 3) & CLIM2 (Visvader-a, Tran-a, Tran-b).

Immunogen

Recombinant protein corresponding to mouse CLIM-1.

Application

This Anti-CLIM-1/2 Antibody is validated for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunofluorescence for the detection of CLIM-1/2.

Western Blotting Analysis: A representative lots detected endogenous CLIM1/2 in CHO and murine pituitary gonadotroph Alpha T3 cells, as well as bacterially expressed and in vitro tranlsated recombinant CLIM1/2 (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791; Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunocytochemistry Analysis: A representative lot revealed an inverse correlation between the expression level of endogenous CLIM1/2 and that of its negative regulator RLIM in HEK293, CHO, murine pituitary gonadotroph Alpha T3, and chicken ectodermal cells by dual fluorescent immunocytochemistry (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CLIM1/2 occupancy at the alphaGSU promoter in murine pituitary gonadotroph Alpha T3 cells (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunohistochemistry Analysis: A representative lot detected similar stage-dependent expression patterns between CLIM1/2 and the ubiquitin ligase RLIM in the developing neural tube during early mouse embryogenesis (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
Immunofluorescence Analysis: A representative lot revealed highly co-localized expressions of CLIM1/2 and RLIM on thoracic sections from E12.5 mouse embryos by dual fluorescent immunohistochemistry (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).

Quality

Evaluated by Western Blotting in E16 embryonic mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected CLIM-1/2 in 10 µg of E16 embryonic mouse brain tissue lysate.

Target description

~50/43 kDa observed. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: chicken, hamster, human, mouse. technique(s): ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_034828. UniProt accession no.: O55203. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... LDB2(9079). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.