ANTI-CLOCK (C15-1317-046)

General description

Circadian locomoter output cycles protein kaput (UniProt: O08785; also known as mCLOCK; EC: 2.3.1.48) is encoded by the Clock gene (Gene ID: 12753) in murine species. CLOCK acts as a transcriptional activator that forms a core component of the circadian clock. CLOCK contains 2 PAS domains (aa 107-177 Pas1 domain; aa 262-332 Pas2 domain) and has a nuclear localization signaling sequence (aa 32-47). The major components of circadian clock include CRY proteins, CLOCK or NPAS2, ARNTL/BMAL1 or ARNTL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Transcription and translation of core clock components play a critical role in rhythm generation, whereas delays imposed by post-translational modifications are important for determining the period (tau) of the rhythms. CLOCK forms a heterodimer with ARNTL/BMAL1 and this heterodimer is required for E-box-dependent transactivation and for CLOCK nuclear translocation and degradation. It is also essential for phosphorylation of both CLOCK and ARNTL/BMAL1. CLOCK has an intrinsic acetyltransferase activity, which enables circadian chromatin remodeling by acetylating histones and non-histone proteins, including ARNTL/BMAL1. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 are reported to form the positive limb of the feedback loop, and as a heterodimer activate the transcription of core clock genes and clock-controlled genes. PER1/2/3 and CRY1/2 act as transcriptional repressors and form the negative limb of the feedback loop and inhibit transcription activation. CLOCK is expressed equally in brain, eye, testes, ovaries, liver, heart, lung, and kidney. In the brain, expression is abundant in the suprachiasmatic nuclei, pyriform cortex, and in the hippocampus.

Specificity

This guinea pig polyclonal antibody detects Circadian locomoter output cycles protein kaput (CLOCK) in human and murine cells. It targets an epitope within 39 amino acids from the internal region.

Immunogen

Epitope: unknown

KLH-conjugated linear peptide corresponding to 39 amino acids from the internal region of murine Circadian locomoter output cycles protein kaput (CLOCK).

Application

Anti-CLOCK, Cat. No. ABE2598, is a highly specific guinea pig polyclonal antibody that targets Circadian locomoter output cycles protein kaput and has been tested in Chromatin Immunoprecipitation (ChIP). ChIP-seq, and Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Circadian CLOCK in A431, HUVEC, and NIH/3T3 cells.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CLOCK in beta-TC6 cells (Perelis, M., et. al. (2015). Science. 350(6261):aac4250).

ChIP-seq Analysis: A representative lot detected CLOCK in beta-TC6 cells (Perelis, M., et. al. (2015). Science. 350(6261):aac4250).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CLOCK in HeLa cells.

Target description

96.39 kda calculated.

Physical form

Affinity Purified

Format: Purified

Purified guinea pig polyclonal antibody in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: guinea pig. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. packaging: antibody small pack of 25 . μ. L. technique(s): ChIP: suitable (ChIP-seq), immunocytochemistry: suitable. isotype: IgG. NCBI accession no.: NP_031741. UniProt accession no.: O08785. target post-translational modification: unmodified. Gene Information: mouse ... Clock(12753). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.