Anti-Cofilin-1 Antibody (C15-1318-001)

General description

Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

Specificity

This rabbit polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in HeLa and NIH/3T3 cells.

Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated cofilin-1 from 0.5 mg of MCF-7 cell lysate.

This poylclonal antibody is also available as Alexa Fluor^™ 488 conjugate (Cat. No. ABT1493-AF488) for direct immunofluorescent staining.

Research Category
Cell Structure

This rabbit polyclonal Anti-Cofilin-1 Antibody, Cat. No. ABT1493 has been validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection of cofilin-1.

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cofilin-1 in 10 µg of MCF-7 cell lysate.

Target description

~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB3842

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. species reactivity (predicted by homology): bovine (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology). technique(s): immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_005498. UniProt accession no.: P23528. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... CFL1(1072). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.