Anti-Cohesin subunit SA-2 Antibody (C15-1317-142)

General description

The cohesin subunit SA-2 is a part of the cohesin complex. This complex is comprised of a heterodimer between SMC1 and SMC3 proteins, as well as RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a large ring that can trap sister chromatids after DNA replication. During prophase, the cohesion complexes dissociate from the chromatin due to the phosphorylation by PLK1 (Polo-like kinase) with the exception at the centromeres where the cohesion complexes remain. Then during anaphase, the RAD21 subunit is cleaved which causes the dissociation of the complex from chromosomes, allowing chromosome separation.

Specificity

Other homologies: Mouse and Rat (93%-14/15aa).

Immunogen

KLH-conjugated linear peptide corresponding to human Cohesin Subunit SA-2.

Application

Detect Cohesin subunit SA-2 using this Anti-Cohesin subunit SA-2 Antibody validated for use in 0.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cohesin Subunit SA-2 in A431 and HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blot in HeLa cell nuclear extract.

Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract.

Target description

~140 kDa observed. An uncharacterized band may appear at ~35 kDa in some lysates. Uncharacterized bands may appear at ~40 kDa and ~30 kDa in Immunoprecipitation.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): equine (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_006594. UniProt accession no.: Q8N3U4. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... STAG2(10735). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.