Specificity
Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Immunogen
Human placental collagen type I
Application
Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.
Dot and slot blotting: 1:500-1:1,000
ELISA: 1:1,000-4,000
Indirect immunohistochemistry (frozen sections only): 1:20-1:40
The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Physical form
Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: goat. Quality Level: 100. conjugate: biotin conjugate. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: bovine, human. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, western blot: suitable. suitability: not suitable for immunohistochemistry (Paraffin). NCBI accession no.: NM_000088.3. UniProt accession no.: P02452. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... COL1A1(1277). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB758B
