Specificity

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Immunogen

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

Application

Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.

Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose^™ 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a trademark of Cytiva

biological source: goat. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, bovine. manufacturer/tradename: Chemicon^®.. technique(s): ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. suitability: not suitable for immunohistochemistry (Paraffin). NCBI accession no.: NP_000079. UniProt accession no.: P02452. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... COL1A1(1277). Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H360FD. Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501. Hazard Classifications: Repr. 1B. Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.