Anti-Collagen Type I Antibody (C15-1317-963)

General description

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Immunogen

Rat tail collagen

Application

Detect Collagen Type I using this Anti-Collagen Type I Antibody validated for use in Western Blotting, IHC(P), ELISA.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Specificity ELISA Analysis: A representative lot detected mouse and rat collagen but not human collagen in ELISA.

Western Blot Analysis: A 1:2,000 dilution from a representative lot detected Collagen Type 1 on 10 µg of rat skin tissue lysate.

Quality

Evaluated by Immunohistochemistry in rat skin tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Collagen Type 1 in rat skin tissue.

Target description

130 kDa calculated

Linkage

Replaces: AB755P

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat skin tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat. technique(s): ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_445756. UniProt accession no.: P02452. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... COL1A1(1277). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.