Anti-Collagen Type IV Antibody (C15-1316-442)

Specificity

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Immunogen

Human and bovine placental collagen type IV

Application

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.

Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose^™ 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.

ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: goat. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, bovine, mouse. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable. suitability: not suitable for Western blot, not suitable for immunohistochemistry (Paraffin). NCBI accession no.: NM_001845.4. UniProt accession no.: P02462. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... COL4A1(1282). Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H360FD. Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501. Hazard Classifications: Repr. 1B. Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.