Anti-Complex I-75kD Antibody (C15-1317-536)

General description

Complex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).

Immunogen

KLH-conjugated linear peptide corresponding to human Complex I-75kD.

Application

Detect Complex I-75kD using this rabbit polyclonal antibody, Anti-Complex I-75kD Antibody validated for use in western blotting & IHC (Paraffin).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Complex I-75kD in 10 µg in human brain and human heart tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Complex I-75kD in human kidney and human cardiac myocytes tissue.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Complex I-75kD in 10 µg of mouse brain tissue lysate.

Target description

~75 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. species reactivity (predicted by homology): bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), orangutan (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_004997. UniProt accession no.: P28331. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NDUFS1(4719). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.