Anti-Connexin 40 Antibody (C15-1315-876)

General description

Mouse Connexin 40 is a 358 amino acid gap junction protein with a predicted M.W. of ~40 kDa. It is prominently expressed in lung, heart and skin (see reviews: Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer , 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994). One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

Specificity

Reactivity with other species has not been determined.

Reacts with mouse connexin 40.

Immunogen

Anti-Connexin 40 is made against a 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of mouse C×40 (2). Peptide sequence is conserved in human and rat Cx40 (100%), chicken Cx42 (77%), human Cx50 (87%), rat Cx50 (81%) and sheep Cx49 (87%).

Application

Anti-Connexin 40 Antibody is an antibody against Cx40 for use in IHC & WB.

Immunohistochemistry Analysis: An 1:50 dilution of a representative lot detected Connexin 40 immunoreactivity associated with the gap junction between the cardiac myoepithial cells in mouse heart tissue sections.
Western Blot Analysis: An 1:1000 dilution of a representative lot detected Connexin 40 in human (HEK293, HeLa, HepG2, HUVEC, Jurkat) and murine (C2C12, HIN/3T3) cell lysates.
Western Blot Analysis: An 1:000 dilution of a representative lot detected Connexin 40 in human, mouse, and rat heart tissue homogenates, as well as in mouse brain tissue homogenates.

Optimal working dilutions must be determined by end user.

Quality

Evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: A 1:1000 dilution of this lot detected Connexin 40 in 10 μg of Jurkat lysates.

Target description

40 kDa

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 15 mM NaCl containing 0.05% sodium azide.

Analysis Note

Control
Placenta lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_005257. UniProt accession no.: P36382. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... GJA5(2702). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.