ANTI-CONNEXIN 43 N-TERMINAL CLONE P1E11.B19

General description

Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.

Specificity

Clone P1E11.B19 detects connexin-43 in most mammalian species. It targets an epitope with in the first 20 amino acids from the N-terminal end.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the first 20 amino acids from the N-terminal region of rat connection-43.

Application

Research Category
Cell Structure

This mouse monoclonal Anti-Connexin 43, N-terminal, clone P1E11.B19, Cat. No. MABT903 is tested for use in Immunocytochemistry and Western Blotting for the detection of Connexin-43.

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem. 408(3):375-85).

Western Blotting Analysis (WB): A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Kalcheva, N., et. al. (2007). Proc Natl Acad Sci USA. 104(51):20512-6).

Western Blotting Analysis: 1-4 µg/mL from a representative lot detected Connexin 43 in 10 µg of rat and mouse heart tissue lysates.

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Gellhaus, A., et. al. (2004). J Biol Chem. 279(35):36931-42).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Goldberg, G.S., et. al. (2002). J Biol Chem. 277(39):36725-30).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).

Quality

Evaluated by Western Blotting in human heart tissue lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 43 in 10 µg of human heart tissue lysates.

Target description

~43 kDa observed; 43.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.