ANTI-CONNEXIN 45 CLONE P3C9

General description

Gap junction gamma-1 protein (UniProt: A4GG66; also known as Connexin-45, Cx45, Gap junction alpha-7 protein) is encoded by the Gjc1 (also known as Gja7) gene (Gene ID: 266706) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-45 is a multi-pass membrane protein with ubiquitous expression. It consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weights diffuse from one cell to a neighboring cell. Connexin 45 is the first cardiac connexin expressed during embryonic development and plays an important role in propagating the action potential from the conduction system to the working myocardium. Connexin 45 gap junction channels are reported to close when the membrane potential becomes negative. This prevents retrograde conduction from myocardium to the conduction system.

Specificity

Clone clone P3C9 detects Connexin-45 in rat and mouse cells. It targets an epitope within 14 amino acids from the C-terminal half of rat connexin 45.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of rat connexin 45.

Application

Research Category
Cell Structure

This mouse monoclonal Anti-Connexin 45, clone P3C9, Cat. No. MABT900 is tested for use in Western Blotting for the detection of Connexin-45.

Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Manias, J.L., et. al. (2008). Cardiovasc Res. 80(3):385-95).

Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Kopanic, J.L., et. al. (2013). Front Pharmacol. 4:106).

Quality

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 45 in 10 µg of mouse heart tissue lysate.

Target description

~45 kDa observed; 45.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.