ANTI-COREST ANTIBODY

General description

The CoREST gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.

Specificity

Recognizes CoREST.

Immunogen

GST fusion protein corresponding to residues 109-293 of human CoREST.

Application

Detect CoREST with Anti-CoREST Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ChIP, ICC, IHC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

routinely evaluated by immunoblot in RIPA lysates from A431, 3T3/A31, Jurkat,and Hela cells

Target description

66 kDa

Physical form

Format: Purified

Protein A Purified immunoglobulin in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, and 0.05% sodium azide as a preservative.

Protein A purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.