Anti-Cortactin (KE-20) antibody produced in rabbit

General description

Cortactin is an F-actin binding protein. It belongs to a highly conserved family of perimembrane signaling protein. Cortactin contains a unique multi-domain structure consisting of 6.5 tandem repeats of a 37-amino acid sequence required for direct binding to F-actin, followed by an α-helix region, a proline-rich region, and three tyrosine residues targeted by Src related kinases. The C-terminal region contains a Src-homology 3 (SH3) domain.

Cortactin is encoded by the CTTN gene on chromosome 11 in humans. It is expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src. The protein localizes within lamellipodia and at the cell surface. Cortactin is also called as EMS1.

Immunogen

synthetic peptide corresponding to the mid-region of human cortactin (amino acids 361-380) conjugated to KLH.

Application

Anti-Cortactin (KE-20) antibody produced in rabbit is suitable for indirect immunofluorescence at a dilution of 1:500 using mouse fibroblasts NIH3T3 cell line and western blotting at a dilution of 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of the human epidermoid carcinoma A431 cell line.

Biochem/physiol Actions

Cortactin is a cytoskeleton protein that facilitates the assembly of cortical actin. It is activated by phosphorylation of tyrosine kinases or serine/threonine kinases. It plays an important role in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis. Cortactin regulates the interactions between components of adherens-type junctions and helps in organizing the cytoskeleton, cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. It acts as a regulator in invasion and migration of tumor cells. Overexpression of cortactin causes osteosarcoma carcinogenesis and along with FAK increases the risk of oral premalignant lesions.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.