General description
Recombinase cre (UniProt P06956) is encoded by the cre gene (Gene ID 2777477) of bacteriophage P1. Cre (Causes recombination with Cyclization recombinase) is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each binds to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals vivo.
Specificity
Clone 5B4.1 detects an epitope outside the N-terminal region.
Immunogen
GST-tagged recombinant Cre lacking the N-terminal region sequence.
Application
Anti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in recombinant Cre.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected 250 ng of recombinant Cre (with added N- and C-terminal tag sequences).
Target description
38.54 kDa calculated.
Linkage
Replaces: MABE3120
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- UPC:
- 41105514
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABE644
