ANTI-CREB-1 ANTIBODY (C15-1317-196)

General description

Cyclic AMP-responsive element-binding protein (CREB) is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2.

Specificity

This antibody recognizes the N-terminus of CREB-1.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human CREB-1.

Application

Detect CREB using this rabbit polyclonal antibody, Anti-CREB-1 Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CREB-1 in PC-12 cells.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB-1 in HEK293T cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CREB-1 in 10 µg of A431 cell lysate.

Target description

~51 kDa observed. A doublet has been observed at 48 kDa in some cell lysates (Rutberg, S. E., et al. (1999). Oncogene. 18(8):1569-1579.). Uncharacterized bands may be observed in some cell lysates.

Linkage

Replaces: 04-218

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, rat. species reactivity (predicted by homology): porcine (based on 100% sequence homology), equine (based on 100% sequence homology). technique(s): ChIP: suitable, immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_004370. UniProt accession no.: P16220. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CREB1(1385). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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