Anti-CREB Antibody (C15-1316-002)

General description

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

Specificity

Human cyclic AMP responsive element binding protein-1 (CREB-1, p43). By Western blot it shows a major band at 46 kDa when assayed against a lysate of 1 x 106 Raji B cells. Minor bands at 75 kDa and 43 kDa may be noted. Partial reactivity to CREM-1 protein. May react non-specifically with other proteins.

Immunogen

CREB-1 (p43) peptide corresponding to the N-terminal (aa 5-45) of human protein conjugated to KLH.

Application

Immunoblot

Immunoprecipitation

ELISA

Supershift assay: 0.5-1.0 μL per assay.

Optimal dilutions must be determined by the end user.

Use Anti-CREB Antibody (Rabbit Polyclonal Antibody) validated in ELISA, EMSA, IP, WB to detect CREB also known as CREB-1, cAMP-response Element-binding Protein-1.

Linkage

Replaces: 04-218

Physical form

Format: Purified

Purified immunoglobulin. Liquid (sterile filtered) in 0.02M Potassium Phosphate buffer containing 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse, rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, electrophoretic mobility shift assay: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_004379.2, NM_134442.2. UniProt accession no.: P16220. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CREB1(1385). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.