Anti-CtBP-2 Antibody (C15-1316-774)

General description

C-terminal-binding protein 2 (UniProt P56545; also known as CtBP2) is a nuclear protein encoded by the CTBP2 gene (Gene ID 1488) in human. CtBP1 and CtBP2 are closely related proteins that are encoded by the vertebrate genome and perform both unique and duplicative functions. Upon activation by nicotinamide adenine dinucleotide binding to their dehydrogenase domains, CtBP1 and CtBP2 exert transcriptional repression activity primarily via recruitment of a corepressor complex that consists of histone deacetylases (HDACs) and histone methyltransferases, although CtBPs can also repress transcription through HDAC-independent mechanisms. A critical function of CtBPs lies in the transcriptional repression of pro-apoptotic genes such as Bax, Puma, Bik, and Noxa. CtBP2 is essential for proper development of the nervous system. Acetylation by histone acetyl transferase p300 regulates CtBP2 activity and subcellular localization, while microenvironmental oxygen levels influences the dimerization, acetylation, and thereby the activity of CtBP2 in proliferating neural stem cells (NSCs).

Specificity

Expected to react with spliced variant Ribeye (isoform 2).

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human CtBP-2.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CtBP-2 in human breast cancer cells, human kidney, human ovarian cancer cells, and human prostate cancer cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-CtBP-2 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of CtBP-2 .

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected CtBP-2 in 10 µg of HeLa cell lysate.

Target description

~50 kDa observed. 48.9 kDa (Isoform 1; calculated); 109.2 kDa (Isoform 2/Ribeye; calculated)." ==> ~50 kDa observed. 48.9 kDa (Isoform 1; calculated); 109.2 kDa (Isoform 2/Ribeye; calculated).

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), horse (based on 100% sequence homology), sheep (based on 100% sequence homology), mouse (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_001077383. UniProt accession no.: P56545. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... CTBP2(1488). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.